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KMID : 0360919650080090839
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Journal of the Korean Medical Association
1965 Volume.8 No. 9 p.839 ~ p.847
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FUNGISTATIC ACTIVITIES OF ANTI-TUBERCULOSIS AND ANTI-LEPROSY DRUGS IN VITRO EXPERIMENT
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禹泰河/Woo, Tae Ha
崔大卿/柳駿/Choi, De Kyung/Yoo, Jun
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Abstract
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Mayer and Buu-Hoi suggested that the morphologic relationship between Mycobacteria and fungi may beparalleled by a biochemical relationship and found that a number of recognized antifungal substances exerted strong and specific antimycobacterialactivities.However, antifungal activities of already existinganti-tuberculous and anti-leprosy chemicals were not clearly understood.
The purpose of this study was to clearify the antifungal activities of known anti-tuberculous and anti-leprosy chemicals in vitro screening tests, thus to give a suggestion to the new applition of the known drugs.
The strains of fungi for this study were Micresporum canis 5 strains, M. gypaeum 1 strain, Trichophyton ferrugineum 5 strains, T. rubrum 5 strains,T mentagrophytes 3 strains, T. gypseum 4 strains,Epidermophyton floccosum 5 strains, Sporotrichum schenckii 1 strain and Cryptococcus neoformans 1strain.
The selected drugs for this study were as follows;
a) The sulfone derivatives : DDS (Dapsono), Aceto-sulfone, Sodium sulfoxone, Didrazine and Promine.
b) The thiocarbanilide derivatives: DETC(Dialide)and Ciba 1906.
c) The unsaturated fatty acid : Potassium salts of chaulmoogric acid and recinoleic acid.
d) The anti-tuberculous drugs : Isoniazid, PAS, and Etisul.
e) Others : Sulfamethoxypy ridazine(Lederkyn), Pota-ssium iodide and Griseofulvin.
The media for these study, 5% glucose sabouraud´sager in slant were used. This media provided great erease in micrfscopic and macroscopic observation.
The drugs were dissolved in water or 2:1 alcohole ther mixture. This solution was pipetted into hot liquid Sabouraud´s medium, 50℃, in the proper amount and well mixed. The mixture were pouredinto tubes, and autoclaved for 15 minutes at 13 lb pressure and slanted. The concentration of the each drug in the media were 5, 10, 50, 100, 500, 1,000,2,000, and 4,000 r/ml. acordingly to the chemicals. The control tubes were similarly prepared by addingthe solvent without testing drug.
One loop of a freshly grown subrulture, 3 to 4weeks growth, were carefully removed and thefungal material were finely grounded by mortar and pestle in 1 ml. of distilled water to prepare homogenous fungal suspension. A loop of the homogenized fungal suspension was evenly in oculated to each tube including exprimental and control.
The inocula were cultured in room temperature,22-25℃, for 4 weeks to observe the suppressiveeffects of the tested chemicals.
Results
1. The anti-tuberculrius and anti-leprosy chemicals, except Promine and PAS, had the potency of nhibiting the fungus growth.
2. The most effective drugs among the tested chemicals were the thiocarbanilide derivative, i. e.DETC and Ciba 1906. These two drugs shown complete inhibition of at and above 50 r/ml. and of systemic fungus at and above 1,000 r/ml. This data suggested of new application of these two compounds for the clinical treatment and also to have further potent compounds from the derivatives of thiocarbanilide.
3. Sulfone drugs inhibited the fungus growth completely at and above 1,000 r/ml. for dermatophytes only.
4. The chaulmoogric acid had the potency of inhibiting the fungus growth, also the recinoleic acid had the strong inhibiting ability of fungus growth .This compounds might be useful as a vehicles of antifungal topical agents.
5. The potassium iodide had no effect in vitro testsneither Dermatophytes nor systemic fungi.
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